Molecular biology techniques : a classroom laboratory manual /
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| Hlavní autor: | |
|---|---|
| Další autoři: | , |
| Typ dokumentu: | Kniha |
| Jazyk: | Angličtina |
| Vydáno: |
Amsterdam :
Elsevier,
2012
|
| Vydání: | Third edition |
| Témata: | |
| On-line přístup: | Elektronická verze přístupná pouze pro studenty a pracovníky MU |
| Příbuzné jednotky: | Tištěná verze::
Molecular biology techniques : a classroom laboratory manual. |
Obsah:
- Lab Session 1 Getting Oriented: Practicing with Micropipettes
- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA
- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation
- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product
- Lab Session 5 DNA Ligation and Transformation of Escherichia coli
- Lab Session 6 Colony Hybridization
- Lab Session 7 Characterization of Recombinant Clones: Part 1
- Lab Session 8 Characterization of Recombinant Clones: Part 2
- Lab Session 9 Characterization of Recombinant Clones: Part 3
- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2
- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1
- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2
- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column
- Lab Session 14 Analysis of Purification Fractions
- Lab Session 15 Total RNA Purification
- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1
- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2
- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1
- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2
- Appendix 1 Equipment
- Appendix 2 Prep List
- Appendix 3 Preparation of Competent E. coli Cells
- Appendix 4 Pre-Lab Questions.