Molecular biology techniques : a classroom laboratory manual /

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Hlavní autor: Carson, Sue (Autor)
Další autoři: Miller, Heather B. (Editor), Witherow, D. Scott (Editor)
Typ dokumentu: Kniha
Jazyk:Angličtina
Vydáno: Amsterdam : Elsevier, 2012
Vydání:Third edition
Témata:
On-line přístup:Elektronická verze přístupná pouze pro studenty a pracovníky MU
Příbuzné jednotky:Tištěná verze:: Molecular biology techniques : a classroom laboratory manual.
Obsah:
  • Lab Session 1 Getting Oriented: Practicing with Micropipettes
  • Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA
  • Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation
  • Lab Session 4 Preparation of Insert DNA (egfp) PCR Product
  • Lab Session 5 DNA Ligation and Transformation of Escherichia coli
  • Lab Session 6 Colony Hybridization
  • Lab Session 7 Characterization of Recombinant Clones: Part 1
  • Lab Session 8 Characterization of Recombinant Clones: Part 2
  • Lab Session 9 Characterization of Recombinant Clones: Part 3
  • Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2
  • Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1
  • Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2
  • Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column
  • Lab Session 14 Analysis of Purification Fractions
  • Lab Session 15 Total RNA Purification
  • Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1
  • Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2
  • Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1
  • Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2
  • Appendix 1 Equipment
  • Appendix 2 Prep List
  • Appendix 3 Preparation of Competent E. coli Cells
  • Appendix 4 Pre-Lab Questions.