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150810s2012 ne |||||o|||||||||||eng d |
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|a EIZ
|b 333
|c EBSCO trvale nakupy
|d 2015-06-22
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020 |
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|a 978-0-12-385545-9
|q (electronic bk.)
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035 |
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|a ocn763161431
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035 |
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|a (OCoLC)763161431
|z (OCoLC)776632410
|z (OCoLC)828058575
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040 |
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|a E7B
|b cze
|c E7B
|d OCLCQ
|d CDX
|d NTE
|d CUX
|d REDDC
|d OCLCQ
|d N$T
|d OCLCQ
|d DEBSZ
|d OCLCQ
|d YDXCP
|d OPELS
|d OCLCQ
|d OCLCA
|d OPELS
|d U3G
|d OCLCO
|d WAU
|d VAM
|d OCLCQ
|d OCLCF
|d TPH
|d OCLCO
|d BOD034
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072 |
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7 |
|a 577
|x Biochemie. Molekulární biologie. Biofyzika
|2 Konspekt
|9 2
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080 |
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|a 577.2
|2 MRF
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080 |
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|a 602:577.112
|2 MRF
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100 |
1 |
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|a Carson, Sue
|7 xx0155317
|4 aut
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245 |
1 |
0 |
|a Molecular biology techniques :
|b a classroom laboratory manual /
|c Susan Carson, Heather B. Miller, D. Scott Witherow
|
250 |
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|a Third edition
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264 |
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1 |
|a Amsterdam :
|b Elsevier,
|c 2012
|
300 |
|
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|a 1 online zdroj (xxvi, 200 stran) :
|b ilustrace
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336 |
|
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|a text
|b txt
|2 rdacontent
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337 |
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|a počítač
|b c
|2 rdamedia
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338 |
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|a online zdroj
|b cr
|2 rdacarrier
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505 |
0 |
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|a Lab Session 1 Getting Oriented: Practicing with Micropipettes -- Lab Session 2 Purification and Digestion of Plasmid (Vector) DNA -- Lab Session 3 PCR Amplification of egfp and Completion of Vector Preparation -- Lab Session 4 Preparation of Insert DNA (egfp) PCR Product -- Lab Session 5 DNA Ligation and Transformation of Escherichia coli -- Lab Session 6 Colony Hybridization -- Lab Session 7 Characterization of Recombinant Clones: Part 1 -- Lab Session 8 Characterization of Recombinant Clones: Part 2 -- Lab Session 9 Characterization of Recombinant Clones: Part 3 -- Lab Session 10 Computational Analysis of DNA Sequence from a Positive Clone: Part 2 -- Lab Session 11 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 1 -- Lab Session 12 Expression of Fusion Protein from Positive Clones, SDS-PAGE and Western Blot: Part 2 -- Lab Session 13 Extraction of Recombinant Protein from Escherichia coli Using a Glutathione Affinity Column -- Lab Session 14 Analysis of Purification Fractions -- Lab Session 15 Total RNA Purification -- Lab Session 16 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 1 -- Lab Session 17 Analysis of gst::egfp mRNA Levels by RT-qPCR: Part 2 -- Lab Session 18 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 1 -- Lab Session 19 Analysis of gst::egfp mRNA Levels by Semi-Quantitative RT-PCR: Part 2 -- Appendix 1 Equipment -- Appendix 2 Prep List -- Appendix 3 Preparation of Competent E. coli Cells -- Appendix 4 Pre-Lab Questions.
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533 |
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|a Elektronická reprodukce.
|n Přístup pouze pro oprávněné uživatele
|
650 |
0 |
7 |
|a molekulární biologie
|7 ph115344
|2 czenas
|
650 |
0 |
7 |
|a rekombinantní DNA
|2 czmesh
|
650 |
0 |
7 |
|a proteinové inženýrství
|7 ph291269
|2 czenas
|
650 |
0 |
9 |
|a molecular biology
|2 eczenas
|
650 |
0 |
9 |
|a protein engineering
|2 eczenas
|
650 |
0 |
2 |
|a DNA, Recombinant
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655 |
|
7 |
|a e-knihy online
|2 CZ-BrMU
|
655 |
|
9 |
|a e-books online
|2 eCZ-BrMU
|
700 |
1 |
|
|a Miller, Heather B.
|7 xx0155316
|4 edt
|
700 |
1 |
|
|a Witherow, D. Scott
|7 xx0155315
|4 edt
|
776 |
0 |
8 |
|i Tištěná verze:
|t Molecular biology techniques : a classroom laboratory manual.
|z 978-0-12-385544-2
|
856 |
4 |
1 |
|z Elektronická verze přístupná pouze pro studenty a pracovníky MU
|u https://ezproxy.muni.cz/login?url=https://search.ebscohost.com/login.aspx?authtype=ip&custid=s8431878&lang=cs&profile=eds&direct=true&db=nlebk&AN=453823
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