MicroRNA expression analysis of selected hematological malignancies
MicroRNAs (miRNAs) are short 20-22 nucleotide RNA molecules that function as negative regulators of gene expression in eukaryotic organisms. RNA mediated gene silencing pathways have essential roles in development, cell differentiation, cell proliferation, cell death, chromosome structure and virus...
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| Typ dokumentu: | VŠ práce nebo rukopis |
| Jazyk: | Angličtina |
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2008.
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| On-line přístup: | http://is.muni.cz/th/101627/prif_m/ |
| Shrnutí: | MicroRNAs (miRNAs) are short 20-22 nucleotide RNA molecules that function as negative regulators of gene expression in eukaryotic organisms. RNA mediated gene silencing pathways have essential roles in development, cell differentiation, cell proliferation, cell death, chromosome structure and virus resistance. These highly conserved RNAs regulating gene expression constitute about 3-5% of predicted genes in animals genomes, and 20-30% of protein-coding genes are probably regulated by miRNAs. MiRNAs are associated with RNA-induced silencing complex (RISC), which was identified before to repress the translation of mRNA by RNA interference (RNAi) mechanism. MiRNAs associate with RISC and bind to target mRNAs through partly complementary sequences and repress their translation. It results in the decresed level of the protein encoded by the mRNA. A single miRNA can bind multiple genes (based on incomplete complementarity) and have a possible profound effect on cell physiology. Moreover, ma. |
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| Popis jednotky: | Vedoucí práce: Šárka Pospíšilová. |
| Fyzický popis: | 56 l. : il. |