Heterologous expression of K39T/K94T cryptogein in Pichia pastoris system
Pichia pastoris je eukaryotická kvasinka, schopná post translačních úprav proteinů, což je velká výhoda oproti běžně v biotechnologiích využívané E. coli. Táto kvasinka je schopna metabolizovat metanol a její gen zodpovědný za expresi alkoholoxydázy (AOX1) je zároveň silný inducibilný promotor. Prot...
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| Typ dokumentu: | VŠ práce nebo rukopis |
| Jazyk: | Angličtina |
| Vydáno: |
2012
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| Témata: | |
| On-line přístup: | http://is.muni.cz/th/356735/prif_b/ |
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| 100 | 1 | |a Sándor, Roman |% UČO 356735 |* [absolvent PřírF MU] |4 dis | |
| 242 | 1 | 0 | |a Heterologní exprese cryptogeinu K39T/K94T v systému kvasinky Pichia pastoris |y cze |
| 245 | 1 | 0 | |a Heterologous expression of K39T/K94T cryptogein in Pichia pastoris system |h [rukopis] / |c Roman Sándor |
| 260 | |c 2012 | ||
| 300 | |a 39 l. : |b il. | ||
| 500 | |a Vedoucí práce: Jan Lochman | ||
| 502 | |a Bakalářská práce (Bc.)--Masarykova univerzita, Přírodovědecká fakulta, 2012 | ||
| 520 | 2 | |a Pichia pastoris je eukaryotická kvasinka, schopná post translačních úprav proteinů, což je velká výhoda oproti běžně v biotechnologiích využívané E. coli. Táto kvasinka je schopna metabolizovat metanol a její gen zodpovědný za expresi alkoholoxydázy (AOX1) je zároveň silný inducibilný promotor. Protein, který byl v naší práci exprimován je cryptogein, protein z rodiny elicitinů, produkovaných patogenními oomycétami z rodu Phytophthora. Tento protein je zodpovědný za projevy infekce na tabákovém listě, způsobuje nekrózu napadené tkáně a také indukuje vznik systematické rezistence rostliny. Mutantní forma tohoto cryptogeinu produkována v této bakalářské práci nese označení K39T/K94T. |% cze | |
| 520 | 2 | 9 | |a Pichia pastoris is an eukaryotic yeast, able to do posttranslational modifications of proteins and protein folding in comparison to other microorganism used in biotechnologies, E. coli. This yeast is also able to metabolize methanol and the gene responsible for expression of alcoholoxidase responsible (AOX1) is utilized as strong promoter. The protein expressed in our work is called cryptogein. Cryptogein belongs to family of elicitins, which are produced by fytopathogenic oomycetes from genus Phytophthora. This protein is responsible for symptoms of infection on tobacco leaves, induces necrotic processes in leaves and stimulates development of systematic resistance in the plant. Mutant form of this cryptogein produced in this work is called K39T/K94T. |9 eng |
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