Mouse hematology : a laboratory manual /

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Hlavní autoři: McGarry, Michael P., 1942- (Autor), Protheroe, Cheryl A., 1963- (Autor), Lee, James J., 1958- (Autor)
Typ dokumentu: Kniha
Jazyk:Angličtina
Vydáno: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2010.
Témata:
Obsah:
  • Collection of peripheral blood
  • Counting red blood cells, platelets, and viable nucleated white blood cells
  • Protocol 1 determination of percent red cell volume (hematocrit)
  • Protocol 2 methodology to determine a red blood cell count using a hemacytometer
  • Protocol 3 methodology to determine a platelet cell count using a unopette and a
  • Hemacytometer
  • Protocol 4 methodology to determine a white blood cell count and viability using a
  • Hemacytometer
  • Peripheral blood films and cytospin preparations
  • Protocol 5 washing slides in preparation for microscopic examination of peripheral
  • Blood and bone marrow
  • Protocol 6 preparation of a peripheral blood film
  • Protocol 7 fixing, staining, and mounting a peripheral blood film
  • Protocol 8 preparation of a peripheral blood suspension for cytospin analysis
  • Cell differential assessments of peripheral blood films
  • Preparation of bone marrow for microscopic examination
  • Protocol 9 bone marrow biopsy of the mouse
  • Protocol 10 preparation of a bone marrow brush smear
  • Protocol 11 preparation of a bone marrow suspension for cytospin analysis
  • Cell differential assessments of bone marrow.