Reporter genes - tool for genotoxicity detection
The RecA test, a luminescence SOS reporter test and two well-known SOS reporter assays, umuC test and SOS chromotest, were compared. The main point of this study was to indicate sensitivity of these tests. This all was on the basis of comparison of effects on standard mutagens and environmental samp...
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| Typ dokumentu: | VŠ práce nebo rukopis |
| Jazyk: | Angličtina |
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2007.
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| On-line přístup: | http://is.muni.cz/th/44485/prif_r/ |
| Shrnutí: | The RecA test, a luminescence SOS reporter test and two well-known SOS reporter assays, umuC test and SOS chromotest, were compared. The main point of this study was to indicate sensitivity of these tests. This all was on the basis of comparison of effects on standard mutagens and environmental samples. The results with the selected chemicals showed that the RecA test was less sensitive in a qualitative way in comparison to the umuC test, however its responses were quite comparable with the SOS chromotest. Results suggest that for our choice of environmental samples and chemical standards the UmuC test is optimal for genotoxicity detection in comparison to RecA test and SOS chromotest. |
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| Popis jednotky: | Vedoucí práce: Pavel Čupr. |
| Fyzický popis: | 53 l. |