Ectopic over-expression of the maize β-glucosidase Zm-p60.1 as a molecular tool to probe subcellular compartmentation of cytokinin metabolism
This thesis deals with the subcellular compartmentation of cytokinin metabolism, specifically with the detailed characterization of the response of transgenic tobacco over-expressing the maize beta-glucosidase Zm-p60.1 in response to exogenous zeatin. Analyses were performed on two subcellular varia...
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| Typ dokumentu: | VŠ práce nebo rukopis |
| Jazyk: | Angličtina |
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2005.
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| On-line přístup: | Elektronická verze přístupná pouze pro studenty a pracovníky MU |
| Shrnutí: | This thesis deals with the subcellular compartmentation of cytokinin metabolism, specifically with the detailed characterization of the response of transgenic tobacco over-expressing the maize beta-glucosidase Zm-p60.1 in response to exogenous zeatin. Analyses were performed on two subcellular variants a) the wild-type enzyme where the enzyme is localised in the chloroplast and b) a recombinant version that is retargeted to the vacuole. The results obtained during the work on this thesis served to characterise the use of Zm-p60.1 as a powerful molecular tool to elucidate the complex network of regulation that underlies the subcellular compartmentation of cytokinin metabolism. |
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| Popis jednotky: | Vedoucí práce: Břetislav Brzobohatý. |
| Fyzický popis: | 64 l. |